Growth Environment Research Articles

Optimizing Wound Healing: Examining the Influence of Biopolymers Through a Comprehensive Review of Nanohydrogel-Embedded Nanoparticles in Advancing Regenerative Medicine.

Nanohydrogel wound healing refers to the use of nanotechnology-based hydrogel materials to promote the healing of wounds. Hydrogel dressings are made up of a three-dimensional network of hydrophilic polymers that can absorb and retain large amounts of water or other fluids. Nanohydrogels take this concept further by incorporating nanoscale particles or structures into the hydrogel matrix. These nanoparticles can be made of various materials, such as silver, zinc oxide, or nanoparticles derived from natural substances like chitosan. The inclusion of nanoparticles can provide additional properties and benefits to the hydrogel dressings. Nanohydrogels can be designed to release bioactive substances, such as growth factors or drugs, in a controlled manner. This allows for targeted delivery of therapeutics to the wound site, promoting healing and reducing inflammation. Nanoparticles can reinforce the structure of hydrogels, improving their mechanical strength and stability. Nanohydrogels often incorporate antimicrobial nanoparticles, such as silver or zinc oxide. These nanoparticles have shown effective antimicrobial activity against a wide range of bacteria, fungi, and other pathogens. By incorporating them into hydrogel dressings, nanohydrogels can help prevent or reduce the risk of infection in wounds. Nanohydrogels can be designed to encapsulate and release bioactive substances, such as growth factors, peptides, or drugs, in a controlled and sustained manner. This targeted delivery of therapeutic agents promotes wound healing by facilitating cell proliferation, reducing inflammation, and supporting tissue regeneration. The unique properties of nanohydrogels, including their ability to maintain a moist environment and deliver bioactive agents, can help accelerate the wound healing process. By creating an optimal environment for cell growth and tissue repair, nanohydrogels can promote faster and more efficient healing of wounds.

Effects of Short-Term Nitrogen Additions on Biomass and Soil Phytochemical Cycling in Alpine Grasslands of Tianshan, China

The nitrogen deposition process, as an important phenomenon of global climate change and an important link in the nitrogen cycle, has had serious and far-reaching impacts on grassland ecosystems. This study aimed to investigate the survival adaptation strategies of plants of different functional groups under nitrogen deposition, and the study identified the following outcomes of differences in biomass changes by conducting in situ simulated nitrogen deposition experiments while integrating plant nutrient contents and soil physicochemical properties: (1) nitrogen addition enhanced the aboveground biomass of grassland communities, in which Poaceae were significantly affected by nitrogen addition. Additionally, nitrogen addition significantly influenced plant total nitrogen and total phosphorus; (2) nitrogen addition improved the plant growth environment, alleviated plant nitrogen limitation, and promoted plant phosphorus uptake; and (3) there was variability in the biomass responses of different functional groups to nitrogen addition. The level of nitrogen addition was the primary factor affecting differences in biomass changes, while nitrogen addition frequency was an important factor affecting changes in plant community structure.

Recent Advances in Stimulus-Responsive Nanocarriers for Pesticide Delivery.

In an effort to make pesticide use safer, more efficient, and sustainable, micro-/nanocarriers are increasingly being utilized in agriculture to deliver pesticide-active agents, thereby reducing quantities and improving effectiveness. In the use of nanopesticides, the choice to further design and prepare pesticide stimulus-responsive nanocarriers based on changes in the plant growth environment (light, temperature, pH, enzymes, etc.) has received more and more attention from researchers. Based on this, this paper examines recent advancements in nanomaterials for the design of stimulus-responsive micro-/nanocarriers. It delves into the intricacies of preparation methods, material enhancements, in vivo/ex vivo controlled release, and application techniques for controlled release formulations. The aim is to provide a crucial reference for harnessing nanotechnology to pursue reduced pesticide use and increased efficiency.

Integrating Crop Modeling and Machine Learning for the Improved Prediction of Dryland Wheat Yield

One of the crucial research areas in agricultural decision-making processes is crop yield prediction. This study leverages the advantages of hybrid models to address the complex interplay of genetic, environmental, and management factors to achieve more accurate crop yield forecasts. Therefore, this study used the data of wheat growth environment, crop management, and historical yield in experimental fields in Anding District, Dingxi City, Gansu Province from 1984 to 2021 to construct eight machine learning models and ensemble models. Furthermore, Agricultural Production Systems sIMulator (APSIM), machine learning (ML), and APSIM combined with machine learning (APSIM-ML) were employed to predict wheat yields in 2012, 2016, and 2021. The results show that the APSIM-ML weighted ensemble prediction model, optimized to minimize the MSE, performed the best. Compared to the optimized ML and APSIM models, the average improvements in the RMSE, RRMSE, and MBE for the test years were 43.54 kg/ha, 3.55%, and 15.54 kg/ha, and 93.96 kg/ha, 7.55%, and 104.21 kg/ha, respectively. At the same time, we found that the dynamic flow of water and nitrogen between the soil and crops had the greatest impact on wheat yield prediction. This study improved the accuracy of dryland wheat yield prediction in Gansu Province and provides technical support for the intelligent production of dryland wheat in the loess hilly area.

Single-cell profiling reveals transcriptome dynamics during bovine oocyte growth

BackgroundMammalian follicle development is characterized by extensive changes in morphology, endocrine responsiveness, and function, providing the optimum environment for oocyte growth, development, and resumption of meiosis. In cattle, the first signs of transcription activation in the oocyte are observed in the secondary follicle, later than during mouse and human oogenesis. While many studies have generated extensive datasets characterizing gene expression in bovine oocytes, they are mostly limited to the analysis of fully grown and matured oocytes. The aim of the present study was to apply single-cell RNA sequencing to interrogate the transcriptome of the growing bovine oocyte from the secondary follicle stage through to the mid-antral follicle stage.ResultsSingle-cell RNA-seq libraries were generated from oocytes of known diameters (< 60 to > 120 μm), and datasets were binned into non-overlapping size groups for downstream analysis. Combining the results of weighted gene co-expression network and Trendy analyses, and differently expressed genes (DEGs) between size groups, we identified a decrease in oxidative phosphorylation and an increase in maternal -genes and transcription regulators across the bovine oocyte growth phase. In addition, around 5,000 genes did not change in expression, revealing a cohort of stable genes. An interesting switch in gene expression profile was noted in oocytes greater than 100 μm in diameter, when the expression of genes related to cytoplasmic activities was replaced by genes related to nuclear activities (e.g., chromosome segregation). The highest number of DEGs were detected in the comparison of oocytes 100–109 versus 110–119 μm in diameter, revealing a profound change in the molecular profile of oocytes at the end of their growth phase.ConclusionsThe current study provides a unique dataset of the key genes and pathways characteristic of each stage of oocyte development, contributing an important resource for a greater understanding of bovine oogenesis.

Efficacy of active and passive modified atmosphere packaging on quality preservation and storage life of pomegranate fruit and arils: A review

Pomegranate has nutritional value and health benefits due to its bioactive compounds and antioxidant properties. Fruit consumption is strongly recommended due to its high content of vitamins, fiber, minerals, and polyphenols. Supplying ready-to-eat pomegranate can be a beneficial technique to increase consumption with regard to its nutritional properties. However, maintaining nutritional quality and preventing microbial spoilage is a major challenge. Fruit quality is lost with visible symptoms such as weight loss, shriveling, husk scald, chilling injury, fungal rot, aril color degradation, and off-flavor during long-term storage. Therefore, it is very important to use appropriate strategies to maintain pomegranate whole fruit and aril quality. Gases around the product create a suitable environment for oxidative reactions and aerobic microorganism growth. Therefore, changing the atmosphere around the product can help maintain its quality. One of the effective methods to increase the postharvest life of products is to use modified atmosphere packaging (MAP), which reduces microbial spoilage and chilling injury, preserves the quality, and extends the shelf life by reducing the respiration rate. Modified atmosphere packaging, which uses natural atmospheric components (O2, CO2 and N2), has been widely accepted due to the lack of toxic residues on the product. This review discusses on recent research in terms of MAP application on quality properties and postharvest life of pomegranate fruit and arils during storage.

How Does Airway Surface Liquid Composition Vary in Different Pulmonary Diseases, and How Can We Use This Knowledge to Model Microbial Infections?

Growth environment greatly alters many facets of pathogen physiology, including pathogenesis and antimicrobial tolerance. The importance of host-mimicking environments for attaining an accurate picture of pathogen behaviour is widely recognised. Whilst this recognition has translated into the extensive development of artificial cystic fibrosis (CF) sputum medium, attempts to mimic the growth environment in other respiratory disease states have been completely neglected. The composition of the airway surface liquid (ASL) in different pulmonary diseases is far less well characterised than CF sputum, making it very difficult for researchers to model these infection environments. In this review, we discuss the components of human ASL, how different lung pathologies affect ASL composition, and how different pathogens interact with these components. This will provide researchers interested in mimicking different respiratory environments with the information necessary to design a host-mimicking medium, allowing for better understanding of how to treat pathogens causing infection in these environments.

Research on the Internet Behavior of Teenagers in Xi'an and Its Parental Intervention Mechanism

With the development of modern Internet technology, China's Internet services are playing an increasingly important role in youth theme education and youth growth and development. People's understanding of the growth and development of adolescents is limited to their physical fitness and intellectual development, and less attention is paid to the mental health of adolescents. To this end, this article plans to use a questionnaire survey method to conduct research. Through the survey, we can promote the continued development of the mental health of teenagers, so that more teenagers and parents can get better communication, so that teenagers can have a better growth environment, and more Good healthy growth and happy development.

Re-identification of herbarium specimens through a morphological analysis reveals two species of &lt;i&gt;Murdannia&lt;/i&gt; (Commelinaceae) from South Korea

The previously reported alien plant &lt;i&gt;Murdannia nudiflora&lt;/i&gt; is known to be distributed on Jejudo Island and in inland areas of South Korea. However, the species distributed in the Jejudo Island region exhibits differences in the morphology of the stems, leaves, and seeds compared to those in inland areas. Although &lt;i&gt;M. loriformis&lt;/i&gt; is morphologically similar to &lt;i&gt;M. nudiflora&lt;/i&gt;, it differs in terms of the development of the main stem, the formation of rosettes, and the occurrence of inflorescences that develop from branched stems. The seed testa of &lt;i&gt;M. loriformis&lt;/i&gt; exhibits a radiating striated pattern and is not verrucose. Through the re-identification of specimens of &lt;i&gt;M. nudiflora&lt;/i&gt; and an examination of individuals and images collected in areas of their distribution, it was determined that the individuals in the Jejudo region are &lt;i&gt;M. loriformis&lt;/i&gt;, whereas those distributed inland were found to be &lt;i&gt;M. nudiflora&lt;/i&gt;. This study presents revised descriptions and a taxonomic key for both species, with new Korean names designated based on the characteristics of the distribution areas and surrounding growth environments.

Comparative analysis and evaluation of wild and cultivated Radix Fici Simplicissimae using an UHPLC-Q-Orbitrap mass spectrometry-based metabolomics approach

Radix Fici Simplicissimae (RFS) is widely studied, and is in demand for its value in medicines and food products, with increased scientific focus on its cultivation and breeding. We used ultra-high-performance liquid chromatography quadrupole-orbitrap mass spectrometry-based metabolomics to elucidate the similarities and differences in phytochemical compositions of wild Radix Fici Simplicissimae (WRFS) and cultivated Radix Fici Simplicissimae (CRFS). Untargeted metabolomic analysis was performed with multivariate statistical analysis and heat maps to identify the differences. Eighty one compounds were identified from WRFS and CRFS samples. Principal component analysis and orthogonal partial least squares discrimination analysis indicated that mass spectrometry could effectively distinguish WRFS from CRFS. Among these, 17 potential biomarkers with high metabolic contents could distinguish between the two varieties, including seven phenylpropanoids, three flavonoids, one flavonol, one alkaloid, one glycoside, and four organic acids. Notably, psoralen, apigenin, and bergapten, essential metabolites that play a substantial pharmacological role in RFS, are upregulated in WRFS. WRFS and CRFS are rich in phytochemicals and are similar in terms of the compounds they contain. These findings highlight the effects of different growth environments and drug varieties on secondary metabolite compositions and provide support for targeted breeding for improved CRFS varieties.

Experiment on the Uniformity of Internal Lighting in Zigzag Photovoltaic Greenhouses by Grating Plates

As one of the main projects of facility agriculture promotion, the PV (photovoltaic) greenhouse has the problems of PV power generation competing for light with crop production, strong indoor chiaroscuro, and uneven light distribution. The internal light uniformity is tested by a zigzag greenhouse model to compare the light transmission effects of different light-transmitting materials applied to PV greenhouses. Altogether, 20 line/inch 3 mm and 30 line/inch 3 mm, 40 line/inch 2 mm, 25 line/inch 4 mm grating plates and 2 mm and 3 mm thick ordinary glass were used as light-transmitting components, and the light intensity and light uniformity in the greenhouse were the measurement indicators. The results show that the use of grating plates as covering material can improve the light intensity at the intersection of light and dark, but the overall light transmittance is not as good as glass because it is plastic, which ages easily with low light transmittance. It can also improve the use of land under the shade of PV modules to provide a better growth environment for crops. The test results show that using grating plates can maximize the light intensity of the greenhouse and solve the problem of uneven distribution of light inside the greenhouse caused by obstruction of PV equipment and greenhouse framework. In sunny weather, the light intensity in three rows of the measurement points at the north side in the greenhouse is greater than 20,000 Lx, and the light environment in other areas is between 5000 Lx and 20,000 Lx, which is suitable for planting shade-loving crops.

EDUCATION AND PROFESSIONAL DEVELOPMENT: OPPORTUNITIES AND CHALLENGES FOR IN-SERVICE TEACHERS: A REVIEW

Education and professional development play pivotal roles in enhancing the quality of education and ensuring educators remain adept in an ever -evolving educational landscape for in-service teachers. This paper explores the multifaceted nature of opportunities and challenges faced by in-service teachers in their pursuit of continuous improvements. Emerging teaching methodologies offer in-service teachers novel ways to engage the students and enhance learning outcomes. In-service teachers face time constraints due to their demanding schedules, hindering their ability to fully engage in training &amp; professional development activities. Limited access to up-to-date teaching materials, technology, and professional development resources poses a challenge for in-service teachers striving to provide contemporary and effective education. The rapid evolution of the educational paradigms, curriculum changes &amp; pedagogical shifts can be challenging for in-service teachers to keep pace with, requiring ongoing professional development &amp; adaptability. This paper aims to shed light on delicate balance between the promising opportunities that advancements in education offer and the pragmatic challenges faced by the in-service teachers. Understanding and addressing these dynamics is vital for fostering a supportive and enriching environment for professional growth, ultimately benefiting both educators and the students they serve.

The relationship between childhood trauma andinternet addiction in adolescents: Ameta-analysis.

Many studies have explored the relationship between childhood trauma and internet addiction from different theoretical perspectives; however, the results have been inconsistent. The purpose of this meta-analysis was to examine the relationship between childhood trauma and internet addiction. The PubMed, Web of Science, Embase, CNKI, Wanfang and VIP electronic databases were searched to identify studies examining the correlation between childhood trauma and adolescent internet addiction. The databases were searched from inception to December 31, 2022. Two researchers independently screened the literature, extracted the data, and evaluated the risk of bias of the included studies. Then, Stata 17.0 software was used to perform meta-analysis. This study was registered on PROSPERO (CRD42023388699). A total of 19 studies involving 21,398 adolescents were included in this meta-analysis. The random effects model was used for pooled analysis, and the results revealed a strong positive association between childhood trauma and internet addiction (r = 0.395, 95% CI [0.345, 0.442]). The relationship between childhood trauma and internet addiction was moderated by sample size, survey area, and internet addiction measurement tools. There were significant differences between the associations based on the various child trauma measurement tools and study quality scores. However, interstudy heterogeneity was not significantly affected by study year, sample source, or participant age. Internet addiction is positively correlated with childhood trauma. Therefore, it is extremely important for parents to provide a good growth environment during childhood to enhance the physical and mental development of adolescents. A warm family atmosphere helps individuals develop a healthy personality, thereby reducing or preventing the occurrence of internet addiction. Due to the limited number and low quality of the included studies, the above conclusions need to be verified by additional high-quality studies.

Bone tissue engineering based on sustained release of MiR29c-modified framework nucleic acids from an injectable hydrogel

Bone defects affect the daily activities of patients and are prevalent complications of various skeletal disorders. The miR29 family regulates bone physiology and pathological processes. MiR29c can inhibit Dickkopf-1 (DKK1) expression, up-regulate the Wnt-signaling pathway, and modify the intracellular microenvironment. However, its application is hindered by poor stability and low bioavailability. This study sought to optimize the nano miR29c delivery system, enable enhanced drug effects, stable drug in situ retention, and sustained drug release. Tetrahedral framework nucleic acids (tFNAs), which have excellent drug-loading ability, endocytic capacity, and stability, were used with sticky ends to deliver miR29c (i.e., stFNAs-miR29c). Porous gelatin methacryloyl (GelMA) was selected as a scaffold for the in situ sustained release of stFNAs-miR29c and to provide a growth environment for bone marrow mesenchymal stem cells (BMSCs). This research developed a miR29c sustained-release system in situ: GelMA-stFNAs-miR29c and demonstrated its osteogenesis in vitro and in vivo. GelMA, stFNAs, and miR29c can function synergistically at nano-, micro-, and macro- scales that achieves high stability and efficiency on microRNA nano-delivery, regulates the local microenvironment, maintains the macro-osteogenic space, accelerates the osteogenic process, and ensures excellent osteogenesis effects, contributing to the broader application of miRNAs in biomedicine and improved bone regeneration in situ.

Generation of induced pluripotent stem cells from rat fibroblasts and optimization of its differentiation into mature functional neurons

BackgroundInduced pluripotent stem cells (iPSCs) derived neural stem cells (NSCs) provide a potential for autologous neural transplantation therapy following neurological insults. Thus far, in preclinical studies the donor iPSCs-NSCs are mostly of human or mouse origin with concerns centering around graft rejection when applied to rat brain injury models. For better survival and integration of transplanted cells in the injured brain in rat models, use of rat-iPSC-NSCs and in combination with biomaterials is of advantageous. Herein, we report a detailed method in generating rat iPSCs with improved reprogramming efficiency and differentiation into neurons. New methodRat fibroblasts were reprogrammed into iPSCs with polybrene and EF1α-STEMCCA-LoxP lentivirus vector. Pluripotency characterization, differentiation into neuronal linage cells were assessed with RT-qPCR, Western blotting, immunostaining and patch-clamp methods. Cells were cultured in a custom-designed integrin array system as well as in a hydrogel-based 3D condition. ResultsWe describe a thorough method for the generation of rat-iPSC-NSCs, and identify integrin αvβ8 as a substrate for the optimal growth of rat-iPSC-NSCs. Furthermore, with hydrogel as the supporting biomaterial in the 3-D culture, when combined with integrin αvβ8 binding peptide, it forms a conducive environment for optimal growth and differentiation of iPSC-NSCs into mature neurons. Comparison with existing methodsPublished studies about rat-iPSC-NSCs are rare. This study provides a detailed protocol for the generation of rat iPSC-NSCs and optimal growth conditions for neuronal differentiation. Our method is useable for studies to assess the utility of rat iPSC-NSCs for neural transplantation in rat brain injury models.

Abstract 4357: Examining the role of end-stage kidney as a potential intermediate stage in the development of renal cell carcinomas; understanding the pathogenesis allowing for early detection

Abstract Introduction: Clear cell renal cell carcinoma (CCRCC) and papillary renal cell carcinoma (PRCC) are both thought to arise from the renal proximal tubules. End stage renal disease (ESRD) patients have an increased incidence of developing PRCC and CCRCC. Some studies showed a progenitor cell population that is inherently present in renal tubules, and is significantly increased after renal injury. The studies have also illustrated similarities between these progenitor cells and PRCC &amp; CCRCC; hypothesizing that they are the cell of origin of these tumors. This trend was not observed in other renal cell carcinomas such as chromophobe renal cell carcinoma (chRCC). This study aims to further examine the possible role of ESRD in the development of PRCC and CCRCC to understand this initial step of renal cell carcinoma pathogenesis. Methods: A cohort of n = 39 PRCC, 25 CCRCC, 63 normal, 10 end-stage and 18 chRCC cases were selected. RNA sequencing was performed and differential-gene-expression analysis was conducted between normal, ESRD and tumor using DESeq2 software. Pathway analysis was performed using GSEA. Genes from oncogenic pathways that have been studied to be involved in renal cancer development were selected to perform consensus clustering analysis on Genepattern. Results: Oncogenic and developmental pathways enriched in ESRD are consistently overlapping with that of CCRCC and PRCC tumors. 50% of end-stage developmental pathways are found in both CCRCC and PRCC but only 4.5% in chRCC. 62.4% and 46.4% of end-stage oncogenic pathways are found in CCRCC and PRCC respectively but only 16% in chRCC. Clustering analysis showed ESRD cases clustered together with CCRCC and PRCC groups and not with chRCC. Overall, the ESRD cases exhibited a gene expression profile more closely resembling that of CCRCC and PRCC than chRCC. Conclusion: Our findings support the hypothesis that ESRD provides a favorable environment for tumor growth promoting pathogenesis of both PRCC and CCRCC. The enrichment of the same developmental pathways in both ESRD and the tumors suggests that the progenitor cell population in the renal tubules, mentioned above, could be the origin of both PRCC and CCRCC lesions. Further analysis would allow the uncovering of the pathways that lead to tumor progression from these cells of origin, and can be used to determine biomarkers for early detection of CCRCC and PRCC. This is of particular benefit for those with increased risk as the ESRD patients. Citation Format: Mingyuan Wan, Vincent Castillo, Rola Saleeb. Examining the role of end-stage kidney as a potential intermediate stage in the development of renal cell carcinomas; understanding the pathogenesis allowing for early detection [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 1 (Regular Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(6_Suppl):Abstract nr 4357.

A fatigue life prediction approach for porosity defect-induced failures in directed energy deposited Ti-6Al-4V considering crack growth environment

Porosity defects are the main cause of the fatigue failures of directed energy deposited Ti-6Al-4V. Porosity defect-induced cracks initiate in pseudo-vacuum and switch to air upon reaching the specimen surface. In view of this failure characteristic, this study establishes a fatigue life prediction approach based on the fracture mechanics framework considering crack growth environment. Firstly, high cycle fatigue tests at room temperature under atmospheric conditions and fracture surface observation were carried out to obtain the fatigue lives, key defect parameters and crack initiation types. Then, long and small crack growth tests in air were conducted, and crack growth rate curve in air was fitted by the Hartman-Schijve equation variant. Next, the crack growth data in the fracture surfaces were retrieved by the marker load method, which were compared with the crack growth data in vacuum in the existing literature to determine the long crack growth data in pseudo-vacuum. Small crack growth data in pseudo-vacuum were determined by inverse method. And thus, the crack growth rate curve in pseudo-vacuum was established. Finally, a crack length criterion for environmental transition was proposed to segmentally predict the fatigue lives which were compared with the experimental lives to verify the effectiveness of the approach.

Brazilian Northern biotech startups: an examination of supporting factors for web portal modeling

The purpose of this research project is to determine the elements that would facilitate the establishment of a biotechnology startup help site in the northern part of Brazil. As part of the research, data on startups was gathered, obstacles to growth were identified, and issues faced by the companies were noted. Based on the responses to the questionnaire, conclusions were made on the major difficulties these businesses encountered. The main barrier is found to be the difficulty in acquiring finance; other challenging elements include revenue generation and equipment availability. It is acknowledged that regional concerns, such input availability, and logistics, have a significant impact on the difficulties that local businesses encounter. Recommendations are made based on the challenges found to improve the startup growth environment, hence promoting sustainable innovation and the bioeconomy's development.

Construction and Regulation of the Abscisic Acid Biosynthesis Pathway in Yarrowia lipolytica.

Abscisic acid (ABA) is an important plant hormone with a variety of physiological functions such as regulating plant growth and helping plants to resist an adverse growth environment. However, at present, the ABA yield of heterologous biosynthesis by metabolic engineering is still low for industrial production. Therefore, five Botrytis cinerea genes (bcaba1, bcaba2, bcaba3, bcaba4, and bccpr1) related to ABA biosynthesis were expressed in Yarrowia lipolytica PO1h; its ABA production was 24.33 mg/L. By increasing the copy number of IDI and ERG12S, ERG20YMT, and bcaba3, bcaba1 genes, the yield of ABA was increased to 54.51 mg/L. By locating HMG-CoA reductase and HMG-CoA synthase in mitochondria, acetyl-CoA in mitochondria was converted into mevalonate; this increased the ABA yield to 102.12 mg/L. Finally, in the fed-batch fermentation process with the addition of dodecane, the ABA yield was up to 1212.57 mg/L, which is the highest yield of heterologous production of ABA by metabolic engineering.

A Review of the Influence of Genotype, Environment, and Food Processing on the Bioactive Compound Profile of Red Rice (Oryza sativa L.)

Red rice has achieved a lot of visibility due to its greater amounts of bioactive compounds compared to traditional white rice. The increased recognition of red rice by the industry is a consequence of the expansion of its study in the field of research. The red color of its grains is characteristic of the presence of proanthocyanidins, which is associated with health benefits such as reducing the risk of chronic diseases. In addition, red rice is gluten-free and hypoallergenic, which makes it suitable for celiac or gluten-intolerant patients. However, the contents of phytochemicals can vary with the influence of the adaptability of genotypes to the environment, cultivation practices, abiotic stresses, and industrial processing. In this scenario, one of the challenges is to increase the diversity of red rice products while having a minimum impact on the content of bioactive compounds, mainly flavonoids and phenolic acids. In this review, a complete overview of the importance of pigmented red rice is presented, including the effects of different genotypes, the growth environment, and industrial processing on the bioactive compounds, mainly flavonoids and phenolic acids, in red rice, and the health benefits of its products are described. Studies cited in this review article were found by searching through the Web of Science database from 2013 to 2023. After a detailed and up-to-date search, 36 studies were included in this review article.